| 產品編號 | bsm-51687M |
| 英文名稱 | PU.1/Spi1 Mouse mAb |
| 中文名稱 | 造血轉錄因子PU.1單克隆抗體 |
| 別 名 | 31 kDa Transforming Protein; 31 kDa-transforming protein; Hematopoietic transcription factor PU.1; OF; SFPI1; SPI 1 proto oncogene; SPI A; SPI1_HUMAN; Spleen focus forming virus(SFFV) proviral integration oncogene spi1; Transcription factor PU.1. |
| 研究領域 | 免疫學 轉錄調節因子 b-淋巴細胞 表觀遺傳學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | K8J9 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human SPI1 |
| 亞 型 | IgG1, k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. [provided by RefSeq, Oct 2008] Function: Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing. Subunit: Binds DNA as a monomer. Interacts with CEBPD and NONO (By similarity). Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Subcellular Location: Nucleus. Similarity: Belongs to the ETS family. Contains 1 ETS DNA-binding domain. SWISS: P17947 Gene ID: 6688 Database links: Entrez Gene: 6688 Human SwissProt: P17947 Human |
| 產品圖片 |
Sample:
Lane 1: K562 cell lysates
Lane 2: Daudi cell lysates
Lane 3: THP-1 cell lysates
Primary: Anti-PU.1/Spi1 (bsm-51687M) at 1/500~1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 40 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
