產品編號 | bs-16276R |
英文名稱 | GPIHBP1 Rabbit pAb |
中文名稱 | 高密度脂蛋白結合蛋白1抗體 |
別 名 | Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1; GPI anchored HDL binding protein 1; GPI anchored High Density Lipid binding protein 1; GPI HBP1; GPI-anchored HDL-binding protein 1; GPI-HBP1; GPIHBP1; Hbp1; HDBP1_HUMAN; High density lipoprotein-binding protein 1. |
研究領域 | 腫瘤 心血管 細胞生物 免疫學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
克 隆 號 | |
交叉反應 | Human |
產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 18 kDa |
檢測分子量 | |
細胞定位 | 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human GPIHBP1: 61-150/184 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
GPIHBP1 (glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1) is a capillary endothelial cell protein that provides a platform for LPL-mediated processing of chylomicrons. Consisting of 184 amino acids, GPIHBP1 is a single-pass membrane protein that may be regulated by dietary factors and by PPARγ. Mutations in the gene encoding GPIHBP1 are linked to chylomicronemia syndrome, a rare genetic disorder caused by LPL deficiency and is characterized by enlarged liver and spleen, inflammation of the pancreas, fatty deposits under the skin and possibly deposits in the retina of the eye. Function: Plays a key role in the lipolytic processing of chylomicrons. Subunit: Binds with high affinity to high-density lipoprotein (HDL) (By similarity). Binds to lipoprotein lipase (LPL), chylomicrons and APOA5. Subcellular Location: Cell membrane. Localized at the cell surface. Post-translational modifications: Glycosylation of Asn-78 is critical for cell surface localization and the binding of chylomicrons and lipoprotein lipase (By similarity). Similarity: Contains 1 UPAR/Ly6 domain. SWISS: Q8IV16 Gene ID: 338328 Database links: Entrez Gene: 338328 Human SwissProt: Q8IV16 Human Unigene: 426410 Human |
產品圖片 |
Tissue/cell: human schwannoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPIHBP1 Polyclonal Antibody, Unconjugated(bs-16276R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |