| 產品編號 | bs-13735R |
| 英文名稱 | SHROOM1 Rabbit pAb |
| 中文名稱 | SHROOM1蛋白抗體 |
| 別 名 | Apical protein 2; APXL2; Protein Shroom1; SHRM1_HUMAN; Shroom family member 1; Shroom1. |
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Specific References (1) | bs-13735R has been referenced in 1 publications.
[IF=4.556] Zhihua Zhao. et al. Suppression of SHROOM1 Improves In Vitro and In Vivo Gene Integration by Promoting Homology-Directed Repair. Int J Mol Sci. 2020 Jan;21(16):5821 WB ; Human.
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| 研究領域 | 腫瘤 細胞生物 信號轉導 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Sheep,Cow,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SHROOM1: 121-220/852 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
SHROOM1 is an 852 amino acid protein that contains one ASD1 domain and one ASD2 domain. Localized to both the cytoplasm and the cytoskeleton, SHROOM1 interacts with Actin and is thought to be involved in microtubule assembly during cell elongation, possibly playing a role in the development of the nervous system. Multiple isoforms of SHROOM1 exist due to alternative splicing events. The gene encoding SHROOM1 maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Function: May be involved in the assembly of microtubule arrays during cell elongation. Subcellular Location: Cytoplasm; cytoskeleton. Similarity: Belongs to the shroom family. Contains 1 ASD1 domain. Contains 1 ASD2 domain. SWISS: Q2M3G4 Gene ID: 134549 Database links: Entrez Gene: 134549 Human Entrez Gene: 71774 Mouse Omim: 611179 Human SwissProt: Q2M3G4 Human SwissProt: Q5SX79 Mouse Unigene: 519574 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin) Polyclonal Antibody, Unconjugated (bs-13753R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
