| 產品編號 | bs-13024R |
| 英文名稱 | DNAJC9 Rabbit pAb |
| 中文名稱 | DNAJC9蛋白抗體 |
| 別 名 | DnaJ homolog subfamily C member 9; DnaJ Hsp40 homolog subfamily C member 9; DnaJ protein SB73; DNAJC9; DNJC9_HUMAN; HDJC9; J DOMAIN OF DNAJ LIKE PROTEIN 1; JDD1; SB73. |
| 研究領域 | 細胞生物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 細胞外基質 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DNAJC9: 161-260/260 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The DnaJ family comprises a group of chaperone proteins that contain a J domain and have diverse cellular localization and functions. DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis and are also important mediators of proteolysis and protein degradation. DnaJC9 (DnaJ (Hsp40) homolog, subfamily C, member 9), also designated HDJC9, JDD1 or DnaJ protein SB73, is a 260 amino acid protein found at moderate levels in most tissues with highest expression in the germinal zone of the central nervous system, testis, ovary, renal cortex and fetal liver. A member of the DnaJ family, DnaJC9 contains one N-terminal J domain but lacks the typical G/F and zinc finger regions that are typical of DnaJ family members. DnaJC9 localiizes to nuclei under normal conditions but may be transported to cytoplasm and plasma membrane when exposed to heat shock. Similarity: Contains 1 J domain. SWISS: Q8WXX5 Gene ID: 23234 Database links: Entrez Gene: 23234 Human SwissProt: Q8WXX5 human |
| 產品圖片 |
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- DNAJC9 (bs-13024R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 35 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNAJC9) Polyclonal Antibody, Unconjugated (bs-13024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
