| 產品編號 | bs-8537R |
| 英文名稱 | TYMS Rabbit pAb |
| 中文名稱 | 胸苷酸合成酶抗體 |
| 別 名 | Thymidylate synthase; EC 2.1.1.45; HsT422; MGC88736; TS; TSase; OK/SW-cl.29; TYSY_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TYMS: 65-160/313 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017] Function: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Subunit: Homodimer. Subcellular Location: Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. Similarity: Belongs to the thymidylate synthase family. SWISS: P04818 Gene ID: 7298 Database links: Entrez Gene: 7298 Human Entrez Gene: 22171 Mouse SwissProt: P04818 Human SwissProt: P07607 Mouse 胸苷酸合成酶(thymidylate synthase,TS)是生物體內催化胸苷酸合成所必需的酶,多年來一直作為腫瘤化療的重要靶酶。對TS基因調控機制的研究表明:基因擴增、轉錄、翻譯和翻譯后過程都參與了TS表達的調控。TS可與自身的mRNA結合形成TS-mRNA復合物,使mRNA翻譯受阻,5-氟尿嘧啶(5-fluorouracil,5-FU)等抗代謝藥物可與TS蛋白結合,結合后的復合物不能與TS mRNA作用,導致體內TS的表達升高,是腫瘤細胞產生抗藥性的重要分子機制之一。 |
| 產品圖片 |
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti- Thymidylate synthase (bs-8537R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thymidylate synthase) Polyclonal Antibody, Unconjugated (bs-8537R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
