| 產品編號 | bs-1833R |
| 英文名稱 | Vesicular Acetylcholine Transporter Rabbit pAb |
| 中文名稱 | 囊泡乙酰膽堿通道抗體 |
| 別 名 | Vesicular Acetylcholine Transporter; MGC12716; rVAT; SLC18A3; Solute carrier family 18(vesicular acetylcholine) member 3; Solute carrier family 18(vesicular monoamine) member 3; Solute carrier family 18 member 3; VACht; VAChT; VACHT_HUMAN. |
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Specific References (1) | bs-1833R has been referenced in 1 publications.
[IF=2.894] Shen, Zhou. et al. Bone marrow mesenchymal stem cells therapy on bilateral pelvic nerve crush-induced voiding dysfunction in rats. INT UROGYNECOL J. 2022 Apr;:1-8 IF ; Rat.
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| 研究領域 | 腫瘤 心血管 細胞生物 免疫學 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VAT: 201-300/532 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Vesicular Acetylcholine Transporter (VAChT), (~70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer’s disease and other nervous system disorders. Function: Involved in acetylcholine transport into synaptic vesicles. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Peripheral and central cholinergic nervous systems. Similarity: Belongs to the major facilitator superfamily. Vesicular transporter family. SWISS: Q16572 Gene ID: 6572 Database links: Entrez Gene: 6572 Human Entrez Gene: 20508 Mouse Omim: 600336 Human SwissProt: Q16572 Human SwissProt: O35304 Mouse Unigene: 654374 Human Unigene: 190503 Mouse Unigene: 9987 Rat |
| 產品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Human SH-SY5Y cell lysates
Primary: Anti-Vesicular Acetylcholine Transporter (bs-1833R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
