| 產品編號 | bs-1285R |
| 英文名稱 | ETS1 Rabbit pAb |
| 中文名稱 | 轉錄因子家族ETS1抗體 |
| 別 名 | Avian erythroblastosis virus E26(v ets) oncogene homolog 1; C ets 1 protein; ETS 1; Ets protein; ETS-1; ETS1 protein; ETS1_HUMAN; EWSR 2; EWSR2; FLJ10768; p54; Protein C-ets-1; v ets avian erythroblastosis virus E2 oncogene homolog; v ets avian erythroblastosis virus E26 oncogene homolog 1; v ets erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1. |
| 研究領域 | 腫瘤 免疫學 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat,GuineaPig (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ets1: 321-441/441 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]. Function: Transcription factor. Subunit: Interacts with MAF and MAFB. Interacts with PAX5, the interaction alters DNA-binding properties. Binds to DAXX. Interacts with UBE2I. Subcellular Location: Nucleus. Post-translational modifications: Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity. Ubiquitinated; which induces proteasomal degradation. Similarity: Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain. SWISS: P14921 Gene ID: 2113 Database links: Entrez Gene: 2113 Human Entrez Gene: 23871 Mouse Omim: 164720 Human SwissProt: P13474 Chicken SwissProt: P15062 Chicken SwissProt: P14921 Human SwissProt: P27577 Mouse Unigene: 369438 Human Unigene: 292415 Mouse Unigene: 88756 Rat Ets家族成員在胚胎發生早期的血細胞生成和血管發生及胚胎發育后期的組織發生中發揮重要的調控作用. 成年后Ets在許多組織，如造血組織、血管、腦和中樞神經系統、乳腺、子宮內膜、卵巢、睪丸、腎和肺中均有表達. 由于Ets轉錄因子不同成員在胚胎發育過程中的特異性的表達與其功能關系密切。 Ets家族的重要成員Ets-1的表達與胰腺癌、甲狀腺癌、乳腺癌、胃癌、子宮內膜癌、口腔鱗癌、肝細胞性肝癌(HCC)的發生、轉移有很重要的關系。 |
| 產品圖片 |
Sample:Spleen (Mouse) Lysate at 30 ug
Primary: Anti- ETS1 (bs-1285R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Thymus tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Rat Lymph node tissue lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human U251 cell lysates
Primary: Anti-ETS1 (bs-1285R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa
Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ETS1 Polyclonal Antibody, Unconjugated(bs-1285R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A431. Primary Antibody (green line): Rabbit Anti-ETS1 antibody (bs-1285R) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
